COMPLETED FORM 5A's
Form 5A for a project where the SRC has determined a Designated
Supervisor is REQUIRED.
Form 5A for a project where the SRC has determined a Veterinarian
and Designated Supervisor are REQUIRED.
Form 5A for a project where the SRC has determined a Veterinarian,
a Designated Supervisor and Qualified Scientist are REQUIRED.
COMPLETED FORM 5B's
Form 5B for a project that was done at a Regulated Research
Tips for Forms & Projects:
Vertebrate Animal research includes projects involving:
- Live, non-human vertebrate mammalian embryos for fetuses,
- Bird and reptile eggs within three days (72 hours) of hatching;
- All other non-human vertebrates (including fish) at hatching
A project that involves behavioral observations and where:
- there is no interaction with the animals being observed;
- there is no manipulation of the environment in any way; AND
- all federal or state fish, game and wildlife laws and regulations
does NOT need to be reviewed by an SRC prior to experimentation.
The Research Plan for a project that uses Vertebrate Animals must
include the following:
- A discussion of potential alternatives to the use of vertebrate
animals and detailed justification as to why vertebrate animals
must be used.
- An explanation of the potential impact or contribution this
research may have.
- Detail all procedures to be used, including methods used to
minimize potential discomfort, distress, pain and injury to the
animals during the course of experimentation and detailed chemical
concentrations and drug dosages if applicable.
- Detail the number of animals being used, the species, strain,
sex, age, etc. including justification for the number of animals
planned for the research.
- Describe housing and oversight of daily care.
- Discuss disposition of the animals at the termination of the
Vertebrate Animal research projects are categorized into two categories,
those that can be done at a non-regulated site and those that must
be done at a regulated research institution. Vertebrate Animal studies
may be conducted at a non-regulated research site (home, school,
farm, ranch, in the field, etc.) ONLY if each of the following applies:
- The research involves agricultural, behavioral, observational
or supplemental nutritional studies on animals AND
- The research involves only non-invasive and non-intrusive methods
that do not negatively affect an animal's health or well-being.
All other studies using vertebrate animals must be conducted at
a regulated research institution.
No vertebrate animal deaths due to experimental procedures are permitted in any group or subgroup.
- Studies that are designed or anticipated to cause vertebreate animal death are prohibited.
- Any death that occurs must be investigated by a veterinarian, the Qualified Scientist or the Designated Supervisor who is QUALIFIED to determine the cause of death. The project must be suspended until the investigation occurs and the results must be documented in writing.
- If death was teh result of the experimental procedures, the study must be terminated and the study will not qualify for competition at any level.
Research Projects at Non-Regulated Sites
The SRC must decide what type of supervision the project requires:
- A Designated Supervisor is required for all Vertebrate animal Projects done at a non-regulated site.
- A Veterinarian is not required for all Vertebrate Animal studies,
but is recommended for experiments that involve supplemental nutrition
and/or activities that would not be ordinarily encountered in
the animal's daily life.
- A Qualified Scientist is not required for all Vertebrate Animal
studies, but may be suggested by the SRC.
5A must be filled out by the researcher for each different type
of animal being used and include the number of animals being used
in the study, the housing and husbandry to be provided, and what
will happen to the animals after experimentation.
Research Projects at Regulated Research Institutes
5B must be filled out by the Qualified Scientist or the Principal
Investigator at the regulated research institution. A copy of the
regulated research institution IACUC approval letter must be attached
to this form. The Qualified Scientist must describe the USDA Pain
Category that the research falls under. The local SRC must also
review the project to certify that the research complies with ISEF
rules. This review should happen before experimentation begins.
Proper euthanasia at the end of experimentation for tissue removal
and/or pathological analysis is permitted. Only the Qualified Scientist
or an institutional representative may perform the euthanasia.
The following types of studies on vertebrate animals are prohibited:
- All induced toxicity studies, such as those using alcohol, acid
rain, insecticide, herbicide, heavy metal, etc.;
- Behavioral experiments involving operant conditioning with adverse
stimuli, mother/infant separation or induced helplessness;
- Studies of pain; and
- Predator/prey experiments.
SRC approval is needed
BEFORE laboratory/data collection begins.
more about ISEF rules regarding Vertebrate